Porphyromonas gingivalis-감염 Ca9-22 구강편평세포암종 세포에서의 상피-간엽전환 관련 유전자 발현 패턴 분석
An Expression Profile of Epithelial-Mesenchymal Transition-Related Genes in Porphyromonas Gingivalis-Infected Ca9-22 Oral Squamous Cell Carcinoma Cells
박대근, 김다정, 이은영, 우복희, 이지혜, 박혜련,
소속 상세정보
박대근 ( Park Dae-Geun ) - 부산대학교 치의학전문대학원 구경병리학교실
김다정 ( Kim Da-Jeong ) - 부산대학교 치의학전문대학원 구강병리학교실
이은영 ( Lee Eun-Young ) - 부산대학교 치의학전문대학원 구경병리학교실
우복희 ( Woo Vok-Hee ) - 부산대학교 치의학전문대학원 구강병리학교실
이지혜 ( Lee Ji-Hye ) - 부산대학교 치의학전문대학원 구경병리학교실
박혜련 ( Park Hae-Ryoun ) - 부산대학교 치의학전문대학원 구강병리학교실
Abstract
Recently chronic inflammation is focused on the association with cancer progression and acquisition of aggressive biologic behaviors, such as invasion, metastasis, and resistance to chemotherapeutic reagents. Due to the close vicinity within oral cavity, oral cancer may be intimately associated with chronic periodontitis. The present study was done to observe the effect of chronic periodontitis on oral cancer cells by utilizing P. gingivalis infection, a major pathogen in chronic periodontitis. We analyzed and compared the mRNA expression levels of epithelial-mesenchymal transition (EMT) markers in non-infected and P. gingivalis-infected oral cancer cells. Eighty-six genes, which are well known as EMT markers, were analyzed using commercially available EMT microarray plates, performed in triplicate.
Among the 86 genes, the expression of 26 was increased (≥ 2 fold) by P. gingivalis, whereas that of 7 genes was decreased (≥ 2 fold). Our study suggests that P. gingivalis infection evokes significant changes in EMT-related genes. Further observations on molecular mechanisms underlying these changes may help to clarify the role of chronic periodontitis on cancer progression and to develop more efficient preventive and therapeutic modalities for oral cancer. (182 words)
키워드
Oral cancer; Chronic periodontitis; Porphyromonas gingivalis; Epithelial-mesenchymal transition
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